Laser Sheet & Fluorescent Dye Reaction

May 18, 2020

Hello, everyone! Welcome back to my blog. In this post, I want to briefly talk about a fundamental mechanism in the experiment I was supposed to conduct at George Mason University. As I mentioned before, the only way I could determine whether the combination of polymer breakdown and focused ultrasonic disruption can increase the permeability of an artificial blood-brain barrier is by observing color changes on a laser sheet below the Transwell microporous membrane. First, however, I think it’s necessary to understand why this particular color change would occur. After doing some literature review, I’ve obtained a better understanding of how fluorescent dyes function. I must first note that the fluorescent dye I was planning to use was sodium fluorescein salt. This dye is a “fluorophore,” meaning that when excited by a certain wavelength of light, it releases photons of a different wavelength as the dye’s electrons return to their ground state. When in contact with a blue laser sheet, for instance, I expect sodium fluorescein salt to release a green light. For now, this is a brief overview of what I know about the chemical reaction. I will continue researching it, and I will let you all know what I find. Until then, I wish everyone all the best. See you all on the next blog!

Sincerely,

Kamil

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